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mouse antibody against human bcl 2  (Santa Cruz Biotechnology)


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    Structured Review

    Santa Cruz Biotechnology mouse antibody against human bcl 2
    CAR <t>regulates</t> <t>Bcl-2</t> and Bax protein expression in cancer cells. The expression levels of Bcl-2 and active Bax proteins were determined in untreated and CAR-treated HCT116 and HeLa cells at its IC 50 value (30 μM), utilizing flow cytometry after a 48 h period. The histogram bars illustrate the mean fold change in protein expression (MFI) ± SEM and the mean fold change in the Bax/Bcl-2 ratio ± SEM ( n = 3). * p < 0.05 and ** p < 0.01 indicate significant levels compared to the control group (Ctrl).
    Mouse Antibody Against Human Bcl 2, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 14098 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+antibody+against+human+bcl+2/Bcl-2/pmc12938579-128-34-39
    Average 96 stars, based on 14098 article reviews
    mouse antibody against human bcl 2 - by Bioz Stars, 2026-10
    96/100 stars

    Images

    1) Product Images from "Antipsychotic Drug Cariprazine Induces Distinct Cell Death Mechanisms in HeLa and HCT116 Cells as a Potential Inhibitor of Qi-Site of Cytochrome bc1 Reductase"

    Article Title: Antipsychotic Drug Cariprazine Induces Distinct Cell Death Mechanisms in HeLa and HCT116 Cells as a Potential Inhibitor of Qi-Site of Cytochrome bc1 Reductase

    Journal: Biomedicines

    doi: 10.3390/biomedicines14020315

    CAR regulates Bcl-2 and Bax protein expression in cancer cells. The expression levels of Bcl-2 and active Bax proteins were determined in untreated and CAR-treated HCT116 and HeLa cells at its IC 50 value (30 μM), utilizing flow cytometry after a 48 h period. The histogram bars illustrate the mean fold change in protein expression (MFI) ± SEM and the mean fold change in the Bax/Bcl-2 ratio ± SEM ( n = 3). * p < 0.05 and ** p < 0.01 indicate significant levels compared to the control group (Ctrl).
    Figure Legend Snippet: CAR regulates Bcl-2 and Bax protein expression in cancer cells. The expression levels of Bcl-2 and active Bax proteins were determined in untreated and CAR-treated HCT116 and HeLa cells at its IC 50 value (30 μM), utilizing flow cytometry after a 48 h period. The histogram bars illustrate the mean fold change in protein expression (MFI) ± SEM and the mean fold change in the Bax/Bcl-2 ratio ± SEM ( n = 3). * p < 0.05 and ** p < 0.01 indicate significant levels compared to the control group (Ctrl).

    Techniques Used: Expressing, Flow Cytometry, Control

    Related Articles

    Expressing:

    Article Title: Antipsychotic Drug Cariprazine Induces Distinct Cell Death Mechanisms in HeLa and HCT116 Cells as a Potential Inhibitor of Qi-Site of Cytochrome bc1 Reductase
    Article Snippet: The cells were subsequently labeled with primary antibodies at a concentration of 1:100.The cells were subsequently labeled with primary antibodies at a concentration of 1:100.. These antibodies included a mouse antibody against human active caspase-3 (# 9661, Cell Signaling, Danvers, MA, USA), a mouse antibody against human Bax (Santa Cruz Biotech, Inc., Dallas, TX, USA, N20, # sc-493), a mouse antibody against human Bcl-2 (Santa Cruz Biotech, Inc., Dallas, TX, USA, DC21, #sc-783), and a rabbit antibody against human phospho-Akt (Ser-473) (#9271, Cell Signaling, Danvers, MA, USA).. Subsequently, the cells were exposed to secondary antibodies, which included goat anti-mouse FITC (1:200), anti-rabbit Alexa Fluor 488 (#4412, Cell Signaling, Danvers, MA, USA) (1:200), and rabbit anti-human SQST1M/p62-Alexa Fluor 488.Subsequently, the cells were exposed to secondary antibodies, which included goat anti-mouse FITC (1:200), anti-rabbit Alexa Fluor 488 (#4412, Cell Signaling, Danvers, MA, USA) (1:200), and rabbit anti-human SQST1M/p62-Alexa Fluor 488.

    Article Title: Antipsychotic Drug Cariprazine Induces Distinct Cell Death Mechanisms in HeLa and HCT116 Cells as a Potential Inhibitor of Qi-Site of Cytochrome bc1 Reductase
    Article Snippet: The cells were subsequently labeled with primary antibodies at a concentration of 1:100.The cells were subsequently labeled with primary antibodies at a concentration of 1:100.. These antibodies included a mouse antibody against human active caspase-3 (# 9661, Cell Signaling, Danvers, MA, USA), a mouse antibody against human Bax (Santa Cruz Biotech, Inc., Dallas, TX, USA, N20, # sc-493), a mouse antibody against human Bcl-2 (Santa Cruz Biotech, Inc., Dallas, TX, https://doi.org/10.3390/biomedicines14020315 USA, DC21, #sc-783), and a rabbit antibody against human phospho-Akt (Ser-473) (#9271, Cell Signaling, Danvers, MA, USA).. Subsequently, the cells were exposed to secondary antibodies, which included goat anti-mouse FITC (1:200), anti-rabbit Alexa Fluor 488 (#4412, Cell Signaling, Danvers, MA, USA) (1:200), and rabbit anti-human SQST1M/p62-Alexa Fluor 488.Subsequently, the cells were exposed to secondary antibodies, which included goat anti-mouse FITC (1:200), anti-rabbit Alexa Fluor 488 (#4412, Cell Signaling, Danvers, MA, USA) (1:200), and rabbit anti-human SQST1M/p62-Alexa Fluor 488.

    Flow Cytometry:

    Article Title: Antipsychotic Drug Cariprazine Induces Distinct Cell Death Mechanisms in HeLa and HCT116 Cells as a Potential Inhibitor of Qi-Site of Cytochrome bc1 Reductase
    Article Snippet: The cells were subsequently labeled with primary antibodies at a concentration of 1:100.The cells were subsequently labeled with primary antibodies at a concentration of 1:100.. These antibodies included a mouse antibody against human active caspase-3 (# 9661, Cell Signaling, Danvers, MA, USA), a mouse antibody against human Bax (Santa Cruz Biotech, Inc., Dallas, TX, USA, N20, # sc-493), a mouse antibody against human Bcl-2 (Santa Cruz Biotech, Inc., Dallas, TX, USA, DC21, #sc-783), and a rabbit antibody against human phospho-Akt (Ser-473) (#9271, Cell Signaling, Danvers, MA, USA).. Subsequently, the cells were exposed to secondary antibodies, which included goat anti-mouse FITC (1:200), anti-rabbit Alexa Fluor 488 (#4412, Cell Signaling, Danvers, MA, USA) (1:200), and rabbit anti-human SQST1M/p62-Alexa Fluor 488.Subsequently, the cells were exposed to secondary antibodies, which included goat anti-mouse FITC (1:200), anti-rabbit Alexa Fluor 488 (#4412, Cell Signaling, Danvers, MA, USA) (1:200), and rabbit anti-human SQST1M/p62-Alexa Fluor 488.

    Article Title: Antipsychotic Drug Cariprazine Induces Distinct Cell Death Mechanisms in HeLa and HCT116 Cells as a Potential Inhibitor of Qi-Site of Cytochrome bc1 Reductase
    Article Snippet: The cells were subsequently labeled with primary antibodies at a concentration of 1:100.The cells were subsequently labeled with primary antibodies at a concentration of 1:100.. These antibodies included a mouse antibody against human active caspase-3 (# 9661, Cell Signaling, Danvers, MA, USA), a mouse antibody against human Bax (Santa Cruz Biotech, Inc., Dallas, TX, USA, N20, # sc-493), a mouse antibody against human Bcl-2 (Santa Cruz Biotech, Inc., Dallas, TX, https://doi.org/10.3390/biomedicines14020315 USA, DC21, #sc-783), and a rabbit antibody against human phospho-Akt (Ser-473) (#9271, Cell Signaling, Danvers, MA, USA).. Subsequently, the cells were exposed to secondary antibodies, which included goat anti-mouse FITC (1:200), anti-rabbit Alexa Fluor 488 (#4412, Cell Signaling, Danvers, MA, USA) (1:200), and rabbit anti-human SQST1M/p62-Alexa Fluor 488.Subsequently, the cells were exposed to secondary antibodies, which included goat anti-mouse FITC (1:200), anti-rabbit Alexa Fluor 488 (#4412, Cell Signaling, Danvers, MA, USA) (1:200), and rabbit anti-human SQST1M/p62-Alexa Fluor 488.

    Control:

    Article Title: Antipsychotic Drug Cariprazine Induces Distinct Cell Death Mechanisms in HeLa and HCT116 Cells as a Potential Inhibitor of Qi-Site of Cytochrome bc1 Reductase
    Article Snippet: The cells were subsequently labeled with primary antibodies at a concentration of 1:100.The cells were subsequently labeled with primary antibodies at a concentration of 1:100.. These antibodies included a mouse antibody against human active caspase-3 (# 9661, Cell Signaling, Danvers, MA, USA), a mouse antibody against human Bax (Santa Cruz Biotech, Inc., Dallas, TX, USA, N20, # sc-493), a mouse antibody against human Bcl-2 (Santa Cruz Biotech, Inc., Dallas, TX, USA, DC21, #sc-783), and a rabbit antibody against human phospho-Akt (Ser-473) (#9271, Cell Signaling, Danvers, MA, USA).. Subsequently, the cells were exposed to secondary antibodies, which included goat anti-mouse FITC (1:200), anti-rabbit Alexa Fluor 488 (#4412, Cell Signaling, Danvers, MA, USA) (1:200), and rabbit anti-human SQST1M/p62-Alexa Fluor 488.Subsequently, the cells were exposed to secondary antibodies, which included goat anti-mouse FITC (1:200), anti-rabbit Alexa Fluor 488 (#4412, Cell Signaling, Danvers, MA, USA) (1:200), and rabbit anti-human SQST1M/p62-Alexa Fluor 488.

    Article Title: Antipsychotic Drug Cariprazine Induces Distinct Cell Death Mechanisms in HeLa and HCT116 Cells as a Potential Inhibitor of Qi-Site of Cytochrome bc1 Reductase
    Article Snippet: The cells were subsequently labeled with primary antibodies at a concentration of 1:100.The cells were subsequently labeled with primary antibodies at a concentration of 1:100.. These antibodies included a mouse antibody against human active caspase-3 (# 9661, Cell Signaling, Danvers, MA, USA), a mouse antibody against human Bax (Santa Cruz Biotech, Inc., Dallas, TX, USA, N20, # sc-493), a mouse antibody against human Bcl-2 (Santa Cruz Biotech, Inc., Dallas, TX, https://doi.org/10.3390/biomedicines14020315 USA, DC21, #sc-783), and a rabbit antibody against human phospho-Akt (Ser-473) (#9271, Cell Signaling, Danvers, MA, USA).. Subsequently, the cells were exposed to secondary antibodies, which included goat anti-mouse FITC (1:200), anti-rabbit Alexa Fluor 488 (#4412, Cell Signaling, Danvers, MA, USA) (1:200), and rabbit anti-human SQST1M/p62-Alexa Fluor 488.Subsequently, the cells were exposed to secondary antibodies, which included goat anti-mouse FITC (1:200), anti-rabbit Alexa Fluor 488 (#4412, Cell Signaling, Danvers, MA, USA) (1:200), and rabbit anti-human SQST1M/p62-Alexa Fluor 488.



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    Image Search Results


    CAR regulates Bcl-2 and Bax protein expression in cancer cells. The expression levels of Bcl-2 and active Bax proteins were determined in untreated and CAR-treated HCT116 and HeLa cells at its IC 50 value (30 μM), utilizing flow cytometry after a 48 h period. The histogram bars illustrate the mean fold change in protein expression (MFI) ± SEM and the mean fold change in the Bax/Bcl-2 ratio ± SEM ( n = 3). * p < 0.05 and ** p < 0.01 indicate significant levels compared to the control group (Ctrl).

    Journal: Biomedicines

    Article Title: Antipsychotic Drug Cariprazine Induces Distinct Cell Death Mechanisms in HeLa and HCT116 Cells as a Potential Inhibitor of Qi-Site of Cytochrome bc1 Reductase

    doi: 10.3390/biomedicines14020315

    Figure Lengend Snippet: CAR regulates Bcl-2 and Bax protein expression in cancer cells. The expression levels of Bcl-2 and active Bax proteins were determined in untreated and CAR-treated HCT116 and HeLa cells at its IC 50 value (30 μM), utilizing flow cytometry after a 48 h period. The histogram bars illustrate the mean fold change in protein expression (MFI) ± SEM and the mean fold change in the Bax/Bcl-2 ratio ± SEM ( n = 3). * p < 0.05 and ** p < 0.01 indicate significant levels compared to the control group (Ctrl).

    Article Snippet: These antibodies included a mouse antibody against human active caspase-3 (# 9661, Cell Signaling, Danvers, MA, USA), a mouse antibody against human Bax (Santa Cruz Biotech, Inc., Dallas, TX, USA, N20, # sc-493), a mouse antibody against human Bcl-2 (Santa Cruz Biotech, Inc., Dallas, TX, USA, DC21, #sc-783), and a rabbit antibody against human phospho-Akt (Ser-473) (#9271, Cell Signaling, Danvers, MA, USA).

    Techniques: Expressing, Flow Cytometry, Control